(Note: DEPC cannot be used with certain buffers. An alternative to DEPC treatment, Invitrogen™ RNA secure™ reagent, is also available and can be used to treat primary amine solutions such as Tris and does not require a 2 hr treatment or autoclaving.ĭEPC treatment is the most commonly used method for eliminating RNase contamination from water, buffers, and other solutions. We provide a variety of Invitrogen™ buffers and water (DEPC-treated or untreated) that are subject to rigorous quality control testing and are guaranteed to be RNase-free. However, certain reagents such as Tris cannot be DEPC treated. Diethylpyrocarbonate (DEPC) treatment is the most common method used to inactivate RNases in water and buffers. We recommend storing the treated equipment in a clearly marked RNase-free zone to prevent accidental contamination.ĭue to the ubiquitous nature of RNases, depending on the source and upkeep of equipment used to provide the water, the water and buffers used in molecular biology applications can be frequent sources of RNase contamination. Mark baked and RNase Zap reagent–treated items as "RNase-free" to distinguish them from untreated pieces. Just make sure that the RNase Zapreagent is not in contact with surfaces of forceps, spatulas, or other reactive metalware (e.g., aluminum) for more than a few minutes, as this could lead to corrosion. When 2 hours is too long to wait, treating with RNase Zap reagent or wipes is a great alternative. Prior to baking, be sure to wrap the metalware items and the tops of beakers and flasks with aluminum foil to prevent contamination after baking. The baking procedure typically involves incubation in an oven at 450☏ for 2 hours or more (don't include tape, as it will burn!). An alternative is to bake these items, which may be more convenient if large numbers of items are involved. When glassware and metalware are required, treat them with Invitrogen™ RNase Zap™ reagent or wipes. Each lot of tips and tubes is rigorously tested for RNase and DNase contamination and is certified nuclease-free. We also supply regular and nonstick microfuge tubes, which can be used for any molecular biology experiment. Invitrogen™ RNase-free tips (including filter tips) of many sizes are compatible with most commonly used pipettors. When working with RNA, always use RNase-free barrier (filter) pipette tips to prevent introduction of RNases or cross-contamination of your RNA samples. We have a broad selection of certified RNase-free tips and tubes. Always use tips and tubes that have been tested and certified RNase-free. Merely autoclaving will not destroy all RNase activity, since these enzymes are very robust and can regain partial activity upon cooling to room temperature. Ensure that tips come from an unopened box and that tubes come from an unopened or carefully handled bag. However, tips and tubes can be an easily overlooked source of potential RNase contamination. High-quality laboratory plastic consumables can generally be considered RNase-free.
Avoid working with RNA where airflow is turbulent or near surfaces that can create particulates (like a chalkboard).
#Face flask dead cells skin#
Bodily fluids (such as perspiration and skin oils) are rich in RNase activity (we jokingly refer to RNases from these sources as "fingerases").
0 kommentar(er)
