#Chlamydomonas reinhardtii snapgene files Activator#
The endogenous and constitutive promoter RbcS2 is combined with an activator (Hsp70A), resulting in increased expression of your gene of interest.Transgene expression from the Chlamydomonas nuclear genome via the pChlamy_4 vector offers several advantages over chloroplast expression, such as posttranslational modifications and protein targeting and/or secretion. Use (optional) seamless assembly to create your constructs.Enable reliable results with exceptional strain viability and purity.Detect and purify your gene of interest with 6His TEV and/or V5-His epitope tags.Select against gene silencing, even over multiple passages.Express up to 1% total soluble protein from your gene of interest.Gibco TAP Growth Medium, offered separately, is optimized for the rapid growth and maintenance of Chlamydomonas. Our Invitrogen GeneArt Chlamydomonas Protein Expression Vector offers transgene expression from the nuclear genome but is optimized for high-level expression, provides selection against gene silencing, and offers dual protein tags for detection and/or purification of your gene of interest.
reinhardtii 137c is a eukaryote with a large genome size of 121 Mb, and a versatile and proven model organism. reinhardtii propagates by vegetative division, the time from initial transformation to product synthesis is significantly reduced relative to plants, requiring as little as 6 weeks to evaluate production at the flask scale, with the potential to scale up to 64,000 L in 4 to 6 weeks.Ĭ.
Green algae are used as the basis for platforms for the production of biofuels and bioproducts, due mainly to their rapid growth and photosynthetic ability.
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